The 37-year-old "Swiss Miss" has retired twice before -- once after testing positive for a metabolite of cocaine -- but she said the ongoing WTA Finals in Singapore will be her last tournament.
"I think now it's definite. It's different, because before I walked away thinking I might come back," Hingis told reporters after her 6-3, 6-2 doubles win alongside Chan Yung-Jan over Anna-Lena Groenefeld and Kveta Peschke.
"After a season like this, I think it's also perfect timing. You know, you want to stop on top and not when you're already going backwards.
"I couldn't ask for a better finish."
Fellow Swiss star Roger Federer credited Hingis with helping him become a record 19-time Grand Slam champion.
"Martina was partially the one who showed me how it was all done," 36-year-old Federer said in Basel where he is playing at the Swiss Indoors tournament.
"It was great for Switzerland to have someone of her calibre. We were very lucky.
"I'm not sad to see her retire, she's been in the game for long enough, she seems at peace with her decision -- that's wonderful. I've always been a fan of her and I always will be."
Hingis could end her career on a high in Singapore, with a semifinal looming against Timea Babos and Andrea Hlavackova. "We have a pretty decent chance. I think we are the ones to beat right now," she said.
Slovakian-born Hingis took the tennis world by storm when she won her first three major titles, the Australian Open, Wimbledon and US Open, in 1997 at the age of just 16, opening a period of dominance when she spent 209 weeks as world number one.
It was a run that inspired a young Roger Federer, the leading light of the group of Swiss players -- also including Stan Wawrinka and Belinda Bencic -- who followed Hingis onto the world stage.
'The time has come'
Hingis, coached by her mother, Melanie Molitor, had five Grand Slam singles titles to her name when injuries drove her into her first retirement in 2003, when she was just 22.
She returned two years later but the comeback was short-lived and Hingis stepped away from the sport again after failing a drugs test at Wimbledon in 2007.
But she has enjoyed considerable success since her reincarnation as a doubles specialist, and ends her career at the top of the rankings and with 20 major titles in doubles and mixed doubles.
"Here we are for the third, and final time," Hingis posted on Facebook. "Looking back now, it's hard to believe that almost exactly 23 years ago I made my professional debut.
"The years that followed have been some of the most rewarding years of my life, both personally and professionally, but I believe the time has come for me to retire, which I will be doing after my last match here in Singapore."
Hingis said she would remain involved in the sport, perhaps in a coaching capacity -- which could raise questions over a potential match-up with Federer.
"I'll always be part of the game of tennis," she said. "Somehow we will be connected. I will definitely take some time out. I have done coaching before. I can help my mom, her tennis school.
"I'm definitely not going to miss that day-in, day-out grind."