Fribourg had been added to the list in early September, but was removed on October 1st.
The western cantons of Geneva and Vaud remain on the list due to high infection rates.
Geneva and Vaud had been added to the list on September 10th in light of high infection rates in the two French-speaking cantons, reported Swiss news outlet 20 Minutes.
The result of the decision is that anyone who enters from either Geneva or Vaud must be tested on arrival in Germany.
This applies to returning German citizens as well as any other arrivals.
The tested person must then quarantine until the test result comes through, relying on health authorities for guidance.
Germany's threshold for placing a country on the high-risk list is 50 infections per 100,000 residents over the previous seven days.
While the infection rates in western Switzerland remain comparatively high, on the whole infection rates are falling.
On Wednesday, Switzerland fell below its self-imposed high-risk threshold.